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. 2022 Oct 25;9:999291. doi: 10.3389/fmolb.2022.999291

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Copyright © 2022 Shi, Wang and Batista.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Iso-PDF Distribution of PPi and primer RNA strands (A,B) Iso-PDF for bases from the first three primer nucleotides, K593, and PPi for RMP (A) and AMP (B) at position i + 3. (C) Close-up view of iso-PDF for PPi (RMP structure: solid; and AMP structure: transparent). (D) The i + 3 RMP complex structure for showing K593, PPi, and 4 nucleotides of the primer strand. (E) Corresponding i + 3 AMP complex structure. (F) Superposition of the two complexes.