TABLE 2.
Specificity | Lectin or antibodya | Reactivityb of apical membranes of:
|
|||||
---|---|---|---|---|---|---|---|
Normal PPc
|
UC PP
|
Normal cecum
|
|||||
M cells | Enterocytese | M cells | Enterocytes | M cells | Enterocytes | ||
l-Fucose | UEA I, AAA, LTA | − | + | + | + | + | + |
OPA | + | + | + | + | + | + | |
d-GalNAc | VVA | − | −/+f | + | + | + | + |
DBA | − | − | +g | +h | − | − | |
SBA | − | −/+f | + | + | + | + | |
HPA | − | −/+f | − | − | − | − | |
WFA, GS I-A4 | − | + | NDd | ND | + | + | |
WBA I | − | − | ND | ND | − | − | |
d-GlcNAc | GS II | − | −/+i | + | + | + | + |
LEA, UEA II | − | −/+f | + | + | + | + | |
STA | −/+f | + | + | + | + | + | |
WGA | − | −/+f | − | − | + | + | |
d-Galactose | Jacalin | + | + | + | + | + | + |
RCA I, GS I-B4 | +/−j | −/+f | + | + | + | + | |
PNA | − | −/+f | + | + | + | + | |
ECA, BPA | − | + | + | + | + | + | |
MAL I | − | − | ND | ND | + | + | |
d-Mannose, d-glucose | LCA | +k | +k | + | + | + | + |
GNA | +k | +k | ND | ND | + | + | |
Sialic acid | LFA, MAL II | − | − | − | − | + | + |
SNA | − | +l | − | +l | + | + | |
Complex | EEA, WBA II | − | − | − | − | + | + |
SJA | − | −/+f | − | −h | − | − | |
Monoclonal antibodies | Anti-blood group A | − | −m | − | − | − | − |
Anti-blood groups B, H-1 | − | − | − | − | − | − | |
Anti-blood group H-2 | − | − | − | − | + | + | |
Anti-Lewis A | + | + | ND | ND | + | + | |
Anti-Lewis X | − | + | ND | ND | + | + | |
Anti-Lewis B | − | +/−n | − | − | + | + | |
Anti-Lewis Y | − | − | − | − | + | + | |
Anti-sialyl Lewis A | + | −o | + | − | +p | − | |
Anti-sialyl Lewis X | − | − | ND | ND | − | − |
See lectin abbreviations and probe specificities in the text.
Differences in reactivities are explained in footnotes.
PP, Peyer’s patch.
ND, not determined.
Refers to villus and FAE enterocytes.
Negative in type O individual, positive in type A individual.
Few (<20%) M cells were stained.
Few villus enterocytes were stained.
Few (<20%) enterocytes were stained in the type A individual.
Positive in type O individual, negative in type A individual.
Not tested on type O individual.
A subpopulation (<90%) of FAE enterocytes was positive; few (<20%) villus enterocytes were stained.
Few (<5%) enterocytes were stained in the type A individual.
Few (<20%) enterocytes were stained in the type O individual.
Few (<20%) FAE enterocytes were positive.
Few (≤5%) M cells were stained.