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. 2022 Oct 25;12:919835. doi: 10.3389/fcimb.2022.919835

Figure 2.

Figure 2

The neutrophil-secreted elastase activity inhibited by rTsSERP1. Neutrophils were induced with 100 nM of fMLP prior to treatment with 40 µM of rTsSERP1or an irrelevant control (rmDHFR) followed by detecting NE activity using a neutrophil elastase substrate for 1 h (A) and the percentage of the NE activity was calculated at 1h by setting neutrophils treated with fMLP alone as 100% activity (B). Media alone was used as a negative control and 0.1 mM of 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) was used as a positive control for serine protease inhibition. The bar charts show the data of three different donors, which are presented as the mean ± SD. The experiments were performed in triplicate with three independent experiments. One-way ANOVA followed by a Bonferroni multiple comparison test were used for statistical analysis: **p < 0.01 and ***p < 0.001. The treatment conditions were included media alone (Mock), only fMLP induction (fMLP), fMLP induction and AEBSF treatment (AEBSF+fMLP), fMLP induction and rTsSERP1 treatment (rTsSERP1+fMLP), fMLP induction and irrelevant control (rmDHFR) treatment (rmDHFR+fMLP).