Figure 4.

rTsSERP1 inhibits neutrophil extracellular traps (NETosis). Neutrophils initially stained with Sytox green (5 µM) were treated with rTsSERP1 or rmDHFR (10 µg/ml) for 30 min followed by induction with PMA (50 nM) for 4 h. NETosis was analyzed by the quantitative fluorescence intensity (A) and fluorescent images (B) with different treatment conditions including media alone (Mock) (a), PMA induction alone (PMA) (b), rTsSERP1 treatment before PMA induction (rTsSERP1+PMA) (c), and treatment with an irrelevant control (rmDHFR) before PMA induction (rmDHFR+PMA) (d). The bar charts show the data of three different donors, which are presented as the mean ± SD. The experiments were performed in triplicate with three independent experiments. One-way ANOVA followed by a Bonferroni multiple comparison test were used for statistical analysis: **p < 0.01 and ***p < 0.001.