Table 2.
Histone H3 peptides tested for binding to various PHD-containing modules
| Construct Peptide |
Pd VIN3123-326 | At VIN3116-307 | At VIN3150-208 | At VIN3130-299 | At OBE1193-272 |
|---|---|---|---|---|---|
| Unmodified H3 (1–21) | ITC & NMR | ITC | ITC & NMR | − | − |
| H3K4me2 (1–15) | ITC & NMR | ITC | ITC & NMR | ITC | ITC |
| H3K9me2 (1–15) | ITC & NMR | ITC | ITC & NMR | − | − |
| H3T3p (1–15) | ITC & NMR | ITC | ITC & NMR | − | − |
| H3S10p (1–15) | ITC & NMR | ITC | ITC & NMR | − | − |
Summary of histone H3 peptides tested with ITC or NMR in this study for binding to Pd or At PHD superdomains or to the minimal At VIN3 PHD finger, each with negative outcome.