Figure 4.

(A) Tracking of HUVECs and hBMSCs on the scaffold. HUVECs and hBMSCs were indicated by red and green fluorescence. hBMSCs were cultured on the scaffold for 12 ​h, and then HUVECs were added. The observations was carried out after incubating HUVECs for 0-5 h; the scale bar was 200 ​μm (B) RT-PCR of HUVECs and hBMSCs on the scaffold. Relative gene expression levels of the angiogenic markers TGF-β, bFGF, CD31, BMP2, VEGF, KDR and OCN. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)