Table 3.
Primer sequences for osteogenic and angiogenic genes.
| Gene | Direction | Sequence (5′-3′) |
|---|---|---|
| GAPDH | Forward | TGTGTCCGTCGTGGATCTGA |
| Reverse | TTGCTGTTGAAGTCGCAGGAG | |
| BMP2 | Forward | TGTGGAGGGTTGTGGGTGT GAA |
| Reverse | TCAACTGGGGTGGGGTTTT | |
| OPN | Forward | TGCAAACACCGTTGTAACCAAAAGC |
| Reverse | TGCAGTGGCCGTTTGCATTTCT | |
| OCN | Forward | AGCAGCTTGGCCCAGACCTA |
| Reverse | TAGCGCCGGAGTCTGTTCACTAC | |
| Col-I | Forward | GCTTGGTCCACTTGCTTGAAGA |
| Reverse | GAGCATTGCCTTTGATTGCTGGTA | |
| bFGF | Forward | GTGTGCTAACCGTTACCTGGCTATGCAAAAGC |
| Reverse | CCAGTTCGTTTCAGTGCCACATTCT | |
| CD31 | Forward | CCGCATATCCAAGGTCAGCA |
| Reverse | CACCTTGGTCCAGATGTGTGAA | |
| eNOS | Forward | CTGAAGGCTGGCATCTGGAA |
| Reverse | CATGTTACTGTGCGTCCACTCTG | |
| KDR | Forward | AGCCAGCTCTGGATTTGTGGA |
| Reverse | CATGCCCTTAGCCACTTGGAA | |
| TGF-β | Forward | TCCTGGCGATACCTCAGCAA |
| Reverse | GCTAAGGCGAAAGCCCTCAA | |
| VEGF | Forward | CATCCAATCGAGACCCTGGTG |
| Reverse | TTGGTGAGGTTTGATCCGCATA |