TYGS GBDP tree showing relationships between isolates and references. Figure was generated by TYGS [30], with genomes sequenced in our hospital (blue + in “User genomes” column), and additional genomes added for context (green +): GCA_000008445.1 and GCF_001758315.1 [19] to reflect B. anthracis which is otherwise absent from the TYGS database, GCF_001619425.1 and GCF_002566425.1 to represent members of B. basilensis sp. nov.. Species clusters are defined by dDDH with a 70% cutoff. Isolates 607738-19, 608121-20, 607095-21, 607632-21 and 403206-21 can be seen to belong to the species B. anthracis, using this standard cut-off (Table 4).