Table 4.
Genomic identification of described Bacillus species. Names of isolates identified genomically as B. anthracis are shown in bold. The isolate name identified as B. basilensis sp. nov. is shown in italics. MLST was performed at https://pubmlst.org/organisms/bacillus-cereus, and rMLST at https://pubmlst.org/species-id. † Where the 16S rRNA gene was found at 14 copies per genome, with 2-6 SNPs between alleles, a representative version was used for the identification. ∗ GTDB warning: Genome has more than 10% of markers with multiple hits.
| Isolate | MLST | 16S rRNA gene identification: top hit(s) against NCBI rRNA database† | rMLST | GTDB | Species by TYGS, with additional genomes |
|---|---|---|---|---|---|
| 128633-19 | 2593 | 1534bp; 100% match to Bacillus cereus NR_115714.1 | B. cereus (50%)/B. thuringiensis (50%) | B. cereus | B. cereus |
| 607738-19 | 75 | 1550bp; 99.74% match to Bacillus fungorum NR_170494.1 | Bacillus (100%) | B. anthracis∗ | B. anthracis |
| 130277-19 | 177 | 1515bp; 100% match to Bacillus cereus NR_074540.1, Bacillus cereus NR_115714.1 | Bacillus (100%) | B. cereus | B. cereus |
| 607730-19 | 2742 | 1550bp; 99.87% match to Bacillus toyonensis NR_121761.1 | B. cereus (50%)/B. thuringiensis (50%) | B. thuringiensis | B. thuringiensis |
| 130415-19 | 8 | 1520bp; 99.87% match to Bacillus fungorum NR_170494.1 | Bacillus (100%) | B. bombysepticus | B. cereus |
| 608121-20 | 62 | 1550bp; 99.74% match to Bacillus fungorum NR_170494.1 | Bacillus (100%) | B. anthracis∗ | B. anthracis |
| 804229-21 | 26 | 1349bp; 100% match to Bacillus tropicus NR_157736.1, Bacillus nitratireducens NR_157732.1, Bacillus luti NR_157730.1, Bacillus albus NR_157729.1 | Bacillus paranthracis (100%) | B. paranthracis∗ | B. paranthracis |
| 607095-21 | 108 | 1550bp; 99.74% match to Bacillus fungorum NR_170494.1 | B. cereus (100%) | B. anthracis | B. anthracis |
| 203778-21 | new combination: 41,6,12,9,4,114,15 | 1550bp; 99.94% match to Bacillus toyonensis NR_121761.1 | Bacillus (100%) | B. thuringiensis | B. paranthracis |
| 402914-21 | 144 | 1538bp; 99.93% match to Bacillus cereus NR_115714.1 | B. cereus (100%) | B. paranthracis∗ | B. paranthracis |
| 129606-21 | 144 | 1302bp; 99.92% match to Bacillus cereus NR_115714.1 | B. cereus (100%) | B. paranthracis∗ | B. paranthracis |
| 607632-21 | 62 | 1550bp; 99.74% match to Bacillus fungorum NR_170494.1 | Bacillus (100%) | B. anthracis∗ | B. anthracis |
| 403206-21 | 1050 | 1550bp; 99.74% match to Bacillus fungorum NR_170494.1 | Bacillus (100%) | B. anthracis | B. anthracis |
| 403291-21 | 26 | 1509bp; 99.93% match to Bacillus cereus NR_115714.1 | Bacillus (100%) | B. paranthracis∗ | B. paranthracis |
| 403507-21 | partial: not all loci found | 1550bp; 99.81% match to Bacillus fungorum NR_170494.1 | B. cereus (100%) | B. thuringiensis | No matching species (B. basilensis sp. nov.) |
| 135455-21 | 784 | 1550bp; 99.94% match to Bacillus toyonensis NR_121761.1 | B. mobilis (100%) | B. mobilis | B. mobilis |