Figure 6.

Testing the predictivity of the model using a therapeutic protein

(A) Steady-state secretion rates in vitro for parathyroid hormone (PTH) as a function of dose compared with GLuc. (B) Equation from model derivation to predict concentration from steady-state rate. Steady-state rate equation from the in vivo scaling model was used along with the elimination rate constant for PTH to predict PTH concentration as a function of dose in mice. (C) Predicted concentrations of PTH with 95% confidence intervals (black line with green dotted line). Pink data points show actual data at two doses which represent mean SD for n = 4 mice/dose. Sum of squares error (Sy.x) shown on graph.