FIGURE 4.

US3(K213A) interacted with UL47. The plasmid of UL47 was co-transfected with US3 or US3 kinase-inactive mutation US3(K213A) in HEK 293T cells. At 36 h post-transfection, cell lysates were performed co-IP assay and western blot assays. Mouse anti-HA or Flag tag antibody was used to precipitate US3 protein or UL47 protein, respectively, and mouse IgG was used as a control group to exclude non-specific binding of UL47 and US3/US3(K213A). (A) UL47 interacted with US3 and US3(K213A). (B) UL47, US3, and US3(K213A) proteins were expressed normally in transfected cells.