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UL47 was phosphorylated by US3 in DPV infection. DEF cells were infected with 0.05 MOI of CHv and ΔUS3, and protein lysates were harvested at 48 hpi to be performed western blot assay. The anti-phospho-PKA substrate antibody was used to detect the phosphorylation of UL47. Anti-UL47 and anti-US3 polyclonal antibodies were used to react with UL47 and US3, respectively. Black arrow indicates phosphorylated UL47.
