TABLE 1.
All primers for plasmid construction.
| Plasmid | Primer sequence (5′-3′) | Product |
| gB-HA/F | CATCATTTTGGCAAAGAATTCGCCACCATG GCCATGTACCGACGGACTATATGT |
gB-HA |
| gB-HA/R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTA ATCTGGAACATCGTATGGGTAAACTCTGTCTGTGACA AGAATT |
|
| Mut47-11/F | AGACAGCGCCGTAGGGCATCAACGCCCAATG | Flag-UL47 (S11A) |
| Mut47-11/R | CCCTACGGCGCTGTCTTCGTGATTTATCCAT | |
| Mut47-12/F | CAGCGCCGTAGGTCAGCAACGCCCAATGGGC | Flag-UL47 (S12A) |
| Mut47-12/R | CTGACCTACGGCGCTGTCTTCGTGATTTATC | |
| Mut47-29/F | TCGGAGGATCGTAGAGCCTCTACCGATCCTT | Flag-UL47 (T29A) |
| Mut47-29/R | CTCTACGATCCTCCGATTTCCTGGCCGACCT | |
| Mut47-30/F | GAGGATCGTAGAACCGCTACCGATCCTTACG | Flag-UL47 (S30A) |
| Mut47-30/R | CGGTTCTACGATCCTCCGATTTCCTGGCCGA | |
| Mut47-42/F | GGAACGTCAAGGAGAGCTGGTAAGAGACGTAC | Flag-UL47 (S42A) |
| Mut47-42/R | GCTCTCCTTGACGTTCCGGCACCGTAAGGATC | |
| Mut47-47/F | AGTGGTAAGAGACGTGCACTTGACAGGACTG | Flag-UL47 (T47A) |
| Mut47-47/R | CACGTCTCTTACCACTTCTCCTTGACGTTCC | |
| Mut47-161/F | CGATCTAGGCGCGAGGCATCTAAACGGGCGC | Flag-UL47 (S161A) |
| Mut47-161/R | CCTCGCGCCTAGATCGTGATATATTGTCACT | |
| Mut47-775/F | TTAAAACGACGTTTGGCTGGTGGGAGGGTAC | Flag-UL47 (T775A) |
| Mut47-775/R | CCAAACGTCGTTTTAATGCTTTTGATGTTGA | |
| Mut47-11/12/F | AGACAGCGCCGTAGGGCAGCAACGCCCAATG | Flag-UL47 (S11A/S12A) |
| Mut47-29/30/F | TCGGAGGATCGTAGAGCCGCTACCGATCCTT | Flag-UL47 (T29A/S30A) |