Figure 1.

Methodological approach to investigate convergence of parameter estimates between shorter (sampling) and longer (reference) recording durations

(A) We include recordings of nuclei neurons and Purkinje cells with an original recording duration of 180 s or longer (see Table 1 for mean recording durations). For each original recording duration, we take 25 reference durations of 120 s, randomly sampled across the full duration of the original recording. For each reference duration, we take 100 sample durations for each of the various lengths (10–120 s), randomly sampled across the full duration of the original recording.

(B) We use one representative reference duration to show the mean difference in parameter estimate of 100 sample durations for each cell included in the analyses. This visualizes which sample durations provide an accurate and precise parameter estimate.

(C) We perform paired t-tests for each reference duration and 100 paired sample durations and count the number of tests with p < 0.05. We repeat this for 25 reference durations and present the mean ± SEM This helps visualize which sample durations provide an accurate parameter estimate.

(D) We count the percentage of cells with a parameter estimate in the sample duration that is within a 10% deviation of the reference duration. This visualizes which sample durations provide a precise parameter estimate.