Figure 2.

Variability in the firing properties of fast-firing neurons in awake mice

(A) Schematic of recording setup for awake mice on a foam running wheel (top). For simplicity, in all schematics, we have not drawn the plates used for head-fixing the mouse or the recording chamber (see STAR Methods for details). Schematic of cerebellar circuit with nuclei neuron recording (bottom).

(B) Two examples of nuclei neuron firing properties. Red traces represent the firing rate calculated over 0.5 s intervals. The black traces show raw electrophysiological recordings. Each vertical line is an extracellularly recorded action potential.

(C–E) Mean difference in (C) firing rate, (D) CV, and (E) CV2 estimate between 100 sample durations and one representative reference duration. Each dot represents the average for 1 cell. Black line represents the mean for all cells included in the analyses.

(F) Number of significant paired t-tests between parameter estimates in 120 s-long reference durations and 100 samples for each sample duration.

(G) Percentage of parameter estimates in 100 samples for each sample duration within 10% deviation of the reference duration.

(H) Number of significant paired t-tests between parameter estimates in 10 s-long reference durations and 100 samples for each sample duration. For F–H: Mean of 25 sets of 1 reference duration with 100 sample durations each = solid line; ± SEM = shaded region. Firing rate is shown in oxblood red, CV in orange, CV2 in pink.