Figure 4.

Variability in the firing properties of cerebellar nuclei neurons and Purkinje cells in anesthetized mice

(A) Schematic of recording setup depicting an anesthetized mouse on a heating pad (left). Simplified schematic of cerebellar circuit (right).

(B) Example trace of a nuclei neuron recording in an anesthetized mouse. Firing rate as calculated over 0.5 s intervals (top) and corresponding raw electrophysiological recording (bottom).

(C) Example trace of a Purkinje cell recording from an anesthetized mouse. Firing rate as calculated over 0.5 s intervals (top) and corresponding raw electrophysiological recording (bottom). Simple spikes are depicted in gray and complex spikes in green.

(D–F) Mean difference in (D) nuclei neuron spike, (E) Purkinje cell simple spike, and (F) Purkinje cell complex spike CV estimates between 100 sample durations and one representative reference duration. Each dot represents the average for 1 cell. Black line represents the mean for all cells included in the analyses.

(G) Number of significant paired t-tests between CV estimates in 120 s-long reference durations and 100 samples for each sample duration.

(H) Percentage of CV estimates in 100 samples for each sample duration within 10% deviation of the reference duration. For G and H: Mean of 25 sets of 1 reference duration with 100 sample durations each = solid line; ± SEM = shaded region. Nuclei neuron all spikes in green; Purkinje cell simple spikes in gray; Purkinje cell complex spikes in green.