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. 2022 Oct 15;12(10):4502–4519.

Figure 6.

Figure 6

IVM inhibited the integrin β1/FAK and Wnt/β-catenin signaling pathways in HCT-8 cells. (A) The activation of Wnt/β-catenin pathway and the expression of the EMT-related proteins and matrix metalloproteinase-9 (MMP9) in HCT-8 cells treated with 6 μM IVM and/or 25 nM vincristine (VCR) for 48 h were determined by Western blotting analysis using GAPDH as internal control. Histone H3 and β-tubulin were used as the nuclear and cytoplasmic fraction marker, respectively. Cells treated with vehicle served as the control. (B-E) The activation of integrin β1/FAK pathway and the activities of p-Paxillin, RhoA, Rac1 and Cdc42 in HCT-8 cells (B and C) and HCT-116 cells (D and E) treated with IVM and/or VCR for 48 h (B and D) or 20 min (C and E) were determined by Western blotting analysis (B and D) and pull-down assay (C and E), respectively. Abbreviations: Lysate, whole cell lysate; Nuc, nuclear lysate; Cyt, cytosolic lysate. The blots shown were representative of two independent experiments.