Figure 5.

SPAG6 mediates the JAK/STAT pathway in LUSC. (A) Factors with significant correlation with SPAG6 expression in LUSC (only the top 25 factors were shown in the heatmap). (B) The pathway affected by SPAG6 analyzed using KEGG pathway enrichment analysis. (C) The mapping of STAT1 and STAT3 luciferase reporter gene plasmids. (D) Effect of SPAG6 on the luciferase activity of STAT1 and STAT3 luciferase reporter gene plasmids by dual-luciferase assays. Data represent mean ± SD from three independent experiments and were analyzed using two-way ANOVA (D) (*P < 0.05). SPAG6, Sperm Associated Antigen 6; LUSC, Lung Squamous Cell Carcinoma.