Table 1.
RT-qPCR primers used in this study
| Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
| SPAG6 | GACAGTAGTGGATGCAGGAGCT | CCATTTCTGCCAGATCCACGGA |
| DNMT3b | TAACAACGGCAAAGACCGAGGG | TCCTGCCACAAGACAAACAGCC |
| Nanog | CTCCAACATCCTGAACCTCAGC | CGTCACACCATTGCTATTCTTCG |
| ALDH1 | CGGGAAAAGCAATCTGAAGAGGG | GATGCGGCTATACAACACTGGC |
| Sox2 | GCTACAGCATGATGCAGGACCA | TCTGCGAGCTGGTCATGGAGTT |
| β-actin | CACCATTGGCAATGAGCGGTTC | AGGTCTTTGCGGATGTCCACGT |
Note: SPAG6, Sperm Associated Antigen 6; DNMT3b, DNA-Methyltransferase 3b; ALDH1, Aldehyde Dehydrogenase 1.