Table 1.
Impact of DMSO on peak areas for processed quality control samples.
| Controla | Peptide | Peak areab | Fold Increase | |
|---|---|---|---|---|
| No DMSO | 2 % DMSO | |||
| Low | Unlabeled | 416.3 | 3151.3 | 7.6 |
| Labeled IS | 1389.7 | 4334.7 | 3.1 | |
| High | Unlabeled | 1572.3 | 6068.0 | 3.9 |
| Labeled IS | 1224.7 | 4470.7 | 3.7 | |
a
Digested QC material (estimated thyroglobulin concentrations 0.6 ng/mL and 7.2 ng/mL) were chromatographically resolved and analyzed with either DMSO infused post-column to mimic 2 % DMSO in the mobile phase or water infused post-column.
b
For unlabeled peptide, peak area reflects the sum of transitions 704.0404 > 586.90, 704.0404 > 586.91, 704.0404 > 586.92; for labeled IS, transitions 707.0300 > 589.89, 707.0300 > 589.90, 707.0300 > 589.91.