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. 2022 Sep 13;26:36–46. doi: 10.1016/j.jmsacl.2022.09.003

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© 2022 THE AUTHORS. Publishing services by ELSEVIER B.V. on behalf of MSACL.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — High spatial resolution IMS (10 µm) collected from a cross-section of an S. aureus biofilm in positive ion mode (A) and negative ion mode (B) showing delineation of three distinct layers. DG(33:0) (m/z 565.520,1/K₀ 1.36, 1.77 ppm error) (and CL(60:0) (m/z 1295.903, 1/K₀ 1.86, 0.46 ppm error) appear to localize primarily to the bottom layer, while DGDG(32:0) (m/z 915.64, 1/K₀ 1.637, 0.55 ppm error) and PIP(43:0) (m/z 1043.653, 1/K₀ 1.68, 3.83 ppm error) localize to the middle layer. Finally, DG(32:0) (m/z 551.504, 1/K₀ 1.34, 1.09 ppm error) and PG(32:0) (m/z 721.502, 1/K₀ 1.35, 0.14 ppm error) are more abundant in the top layer.