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. 2022 Oct 3;149(19):dev200986. doi: 10.1242/dev.200986

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — PLS-DA on LC/MS positive- and negative-mode metabolites identified in mK4 cells upon MEK inhibition. PLS-DA score plot for mass-to-charge ratios of positive (R²X=0.92; R²Y=0.991; Q²Y=0.968; n=1+4 components) (left) and negative (R²X=0.817; R²Y=0.999; Q²Y=0.992; n=1+1 components) (right) detection modes for LC/MS. Each symbol represents the metabolic profile of an individual sample in each group (n=4). Black squares indicate the non-treated group, red triangles indicate the DMSO-treated group and blue diamonds indicate the MEK inhibitor U0126-treated group. In both detection modes, MEK-inhibited cells, in which MAPK/ERK activation is abolished, share the most similar metabolic profile. R²X, total sum of variation in X explained by the model; R²Y, total sum of variation in Y explained by the model, representing the overall quality; Q²Y, predictive accuracy of the model representing the overall cross-validation co-efficiency.