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. 2022 Oct 3;149(19):dev200986. doi: 10.1242/dev.200986

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — MSEA and heatmap visualization. (A) All 46 MAPK/ERK-dependent metabolites identified by LC/MS were applied to MSEA to distinguish the most affected metabolic pathways. The horizontal bar plots are ranked by P-values. The color gradient is an indicator of how strongly a metabolic pathway changes, with the strongest changes being red and weakest changes being white. (B) Heatmap analysis of the changes in mRNA expression contributing to the differential pathways identified by MSEA. The individual differential metabolites are listed in Table S3. Each horizontal row, respectively, provides the data for the transcripts as follows: pentose phosphate pathway: D-ribose, R5P, G6P and ADP; Warburg effect: 3-PG, R5P, citrate, ADP, glutamine, G6P and glutamate; glycine serine metabolism: 3-PG, glycine, methionine, ADP, threonine, glutamate, creatine and ornithinine; purine metabolism: xanthine, R5P, ADP and glutamine; glutathionine metabolism: oxidized glutahione, glycine, glutamate, ADP and pyroglutamic acid; argine and proline metabolism: N-acetyl putrescine, creatine and proline; glutamate metabolism: oxidized glutahione, glutamate, ADP, glutamine, glycine and aspartate; pyrimidine metabolism: uracil, ADP, dihydrothymineand glutamine; urea cycle: aspartate, ornithine, glutamate, ADP and glutamine; ammonia recyling: glutamate, ADP, glutamine, glycine and aspartate. Control, Ctrl; double knockout, Dko; G6P, glucose-6-phosphate; 3-PG, 3-phosphoglyceric acid; R5P, ribose-5-phosphate.