Fig. 7.

Nephron progenitors are prematurely lost in the postnatal kidneys of Pycr1/2 double-knockout mice. (A,B) Representative images of P1 (A) control and (B) Pycr1/2 double-knockout kidneys, which were co-immunostained with cadherin 6 (CDH6, white) to visualize epithelializing nephrons from early pretubular aggregates to S-shaped bodies, and calbindin (CALB, green), highlighting the ureteric bud epithelium. (C,D) Representative images of immunostaining in P1 (C) control and (D) Pycr1/2 double-knockout kidneys in which cyclin D1 (CD1, white) staining indicates differentiation-committed nephron progenitors in the renal vesicles and calbindin (CALB, green) highlights the ureteric bud epithelium. Scale bar: 200 µm. (E-G) Quantification of (E) cadherin 6-positive and (F) cyclin D1-positive differentiating nephrons as well as (G) overall proliferation [phospho-histone H3 (pHH3) was used as a mitotic marker] in control and Pycr1/2 dko kidneys (n=3 kidneys/genotype, 3-5 randomly selected medullar sections were counted). Data are average±s.d. A Mann-Whitney test was used to determine significance.