Table 3.
Integration sites of as intact predicted sakei phages. Listed are the attachment sites attL and attR of each phage, as well as the gene products and the locus tags of the genes where those phages insert
| Phage | attL (Integrase site) | attR (Lysin site) | Insertion site (gene product) | Locus tag |
|---|---|---|---|---|
| TMW 1.1290 P1 | TACCCTACGGACCCTTCCA | TATCCTACAGACCCTTCCA | Fe-S cluster assembly protein SufB | KNO49_00270 |
| TMW 1.46 P2 | TATCCGACGGAGCCTTCCA | TAGCCCACGGACCCTTCCA | Fe-S cluster assembly protein SufB | A4W82_06315 |
| J64 P3 (region 4) | TTATAGGCGTTCGTTTAATT | TTATAGACGTTCATTTAATT | Glucose-6-phosphate isomerase | LSAJ64_RS06935 |
| CBA3635 P1 | TCTATTCCCATTCCACTGTT | TCTATTCCCATTCAATTGTT | Glutamine-hydrolyzing GMP synthase | H3M14_RS01450 |
| ob4.1 P1 | AACAGTGGAATGGGAATAGA | AACAATTGAATGGGAATAGA | Glutamine-hydrolyzing GMP synthase | KIK01_RS00640 |
| J112 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | LSAJ112_RS03875 |
| J64 P2 (region 3) | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | LSAJ64_RS04570 |
| LZ217 P1 | AATTATGGTCAAATT | AATTATGGTCAAATT | Hypothetical protein | CFK76_RS05020 |
| Probio65 P1 | AATTTGACCATAATT | AATTTGACCATAATT | Hypothetical protein | LP065_RS09960 |
| TMW 1.1386 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | KNO52_05825 |
| TMW 1.1393 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | KNO57_04740 |
| TMW 1.46 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | A4W82_04310 |
| WiKim0063 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | LBS_RS02005 |
| WiKim0072 P1 | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | CW750_RS08280 |
| φ-DJ1812 (strain TMW 1.1398) | AATTTGACCATAATTAATTACC | AATTTGACCATAATTTAAAACC | Hypothetical protein | A4W88_04025 |
| TMW 1.23 P1 | AATGGAGCCGGCG | AATGGAGCCGGCG | ssrA (transfer-messenger RNA) | KNO63_02330 |
| FAM18311 P1 | AATGGAGCCGGCG | AATGGAGCCGGCG | ssrA (transfer-messenger RNA) | B4V05_RS06785 |
| C21B P1 | TATGCGCCACCCGGGA | TATGCGCCACCCGGGA | tRNA-Arg | FXV74_RS02010 |
| CBA3635 P2 | TCCCGGGTGGCGCATA | TCCCGGGTGGCGCATA | tRNA-Arg | H3M14_RS08475 |
| DS4 P1 | TATATGCGCCACCCGGGA | TATATGCGCCACCCGGGA | tRNA-Arg | C0213_RS03410 |
| E28G P1 | TAAAATGTCACAGGCG | TAAAATGTCACAGGCG | tRNA-Arg | FX990_RS02215 |
| J54 P1 | ATGCGCCACCCGGGAGT | ATGCGCCACCCGGGAGT | tRNA-Arg | LSAJ54_RS03305 |
| J64 P1 | ATTATGCGCCACCCGGGA | ATTATGCGCCACCCGGGA | tRNA-Arg | LSAJ64_RS03525 |
| MFPB16A1401 P1 | TATGCGCCACCCGGGAGT | TATGCGCCACCCGGGAGT | tRNA-Arg | MFPB16_RS03465 |
| MFPB19 P1 | TAAAATGTCACAGGCG | TAAAATGTCACAGGCG | tRNA-Arg | MFPB19_RS03605 |
| TMW 1.411 P1 | TAAAATGTCACAGGCG | TAAAATGTCACAGGCG | tRNA-Arg | DT321_01900 |
| LK-145 P1 | CCTGCCACGGGCAT | CCTGCCACGGGCAT | tRNA-Leu | CCX78_RS02335 |
| ob4.1 P2 | ATGCCCGTGGCAGG | ATGCCCGTGGCAGG | tRNA-Leu | KIK01_RS07950 |
| TMW 1.1397 P1 | ATGCCCGTGGCAGG | ATGCCCGTGGCAGG | tRNA-Leu | NCX38_RS02535 |