Fig. 5.

Evolutionary conservation of TADs by chromatin state. To assess whether TADs enriched for different chromatin states also vary in terms of evolutionary conservation, we compared conservation ranks between TADs enriched for different chromatin states. A rank of 11 was assigned to TADs with orthologs present in all 11 species, whereas a rank of 1 represents a D. melanogaster-specific TAD. TADs enriched for BLUE and BLACK chromatin have significantly higher levels of conservation than TADs enriched for the YELLOW and RED chromatin states (the Wilcoxon ranked sum test $P<0.045$). Compact letter display: the Wilcoxon rank sum tests were performed for all pairwise comparisons between chromatin states. Chromatin states whose distributions of conservation rank were not significantly different from each other are those that share the same letter above the top whisker of the box.