Fig. 9.

Importance analysis of chromatin states and TAD conservation as predictors of gene expression constraint. We estimated the RI of chromatin state or TAD conservation rank ($X$ axis) for predicting expression constraint. Gray bars show RI (see Methods) calculated from partial least squares regression coefficients (shown above each bar). Coefficients represent expected difference in log2 of the standard deviation in gene expression associated with each variable on the $X$ axis relative to the reference case (YELLOW state and low-conservation). These results suggest that TAD conservation is a relatively weak predictor of gene expression constraint compared with chromatin state.