Data collection parameters for the SPA data set reconstructed in this study.
Magnification | C2 (μm) | Counting rate/dose rate (e−/pix and e−/Å2/s) | Exposure time (s) | Total dose e−/Å2 | Frames per movie | Number of movies | Defocus range (μm) |
---|---|---|---|---|---|---|---|
64k | 50 | 7.6–4.2 | 8 | 33.6 | 40 | 450 | 0.9–2.5 |
81k | 50 | 7.2–6.4 | 5 | 32 | 40 | 1107 | 0.8–2.4 |
130k | 50 | 5.8–13.7 | 2.5 | 34 | 40 | 2295 | 0.5–2 |
130k-EPU | 50 | 12.4–29.3 | 1 | 29.3 | 40 | 9049 | 0.4–1.6 |