Table 1.

Probe wash steps for the HCRv3 protocol.

Step	Solution	Timing
1	25% 5X SSCT/75% probe wash buffer pre-warmed at 37°C	15 min
2	50% 5X SSCT/50% probe wash buffer pre-warmed at 37°C	15 min
3	75% 5X SSCT/25% probe wash buffer pre-warmed at 37°C	15 min
4	100% 5X SSCT pre-warmed at 37°C	15 min
5	100% 5X SSCT at RT	5 min