Table 1.

Primers suggested for typing SARS-CoV-2 VOCs. The Primer melting temperature is based on the use of Takara Primescript III master mix, which contains 2 mM MgCl₂. Primer melting temperature was calculated using the Oligo Analyzer from IDT (https://eu.idtdna.com/calc/analyzer, Integrated DNA Technologies, Coralville, Iowa, USA).

Primer name	Primer sequence	Primer Tm	Primer end position in NC_045512	SpikeSeq mutation analysis range
nCoV-2019_76_LEFT_alt3	GGGCAAACTGGAAAGATTGCTGA	65.4 ºC	22,822	N439 to T716
nCoV-2019_78_RIGHT	TGTGTACAAAAACTGCCATATTGCA	63.9 ºC	238,23