FIG. 1.

Mapping of transposon insertions of biofilm-negative mutants M10 and M11. The flanking DNA sequences of Tn917 are indicated; transpositions at nucleotides 931 and 87 of the icaA gene were accompanied by 5-bp duplications, indicated in bold letters. E, erythromycin resistance gene erm; arrow, direction of transcription of erm. Restriction sites: B, BglII; H, HindIII; X, XbaI; and E, EcoRI. The region containing the transposon insertions in S. epidermidis 9142 is aligned to the homologous icaADBC operon of S. epidermidis RP62A (5, 9).