TABLE 1.
Pretreatment of cells | Phagocytosis (%) ± SD |
---|---|
None | 100 |
Toxin B | |
10 ng/ml | 56 ± 9.0 |
100 ng/ml | 5 ± 4.0 |
Wortmannin (100 ng/ml) | 3 ± 1.5 |
Cytochalasin B (10 μM) | 2 ± 1.0 |
Macrophages were infected with Salmonella (MOI of 25). Phagocytosis was assessed as described in Materials and Methods. Untreated macrophages phagocytosed 55% (± 11) of the original inoculum. The phagocytic activity of untreated macrophages was set as 100%.