FIG. 5.

Comparison of the proliferative responses (top) and IFN-γ production (bottom) of CD8⁺ and CD4⁺ MACS-sorted T cells from animal 02 in response to M. bovis (10⁶ CFU/ml), MBSE, PPDb, and rMPB70 (4 μg/ml). The top panel shows the responses as cpm following [methyl-³H]thymidine incorporation. The bottom panel indicates the IFN-γ production as assessed by ELISA and presented as OD₄₅₀. The proliferative responses obtained for APC alone were similar to the control value. The OD₄₅₀ values obtained for APC alone were <0.2.