FIG. 7.

Effect of BFA-A (left) and CYT-D (right) on the presentation of M. bovis, MBSE, and PPDb. Autologous mitomycin C-treated PBMC were used as APC. The APC were treated with BFA-A (1 μg/ml) or CYT-D (10 μM) for 1 h at 37°C. The different antigens were then added to the cultures, which were further incubated for 1 h. Finally, T-cell clones (CD8⁺ or CD4⁺) were added to the appropriate wells. Results are presented as percentages of the control response, i.e., (response in the presence of chemical [cpm]/response in absence of chemical [cpm]) × 100, and are representative of two repeated experiments with CD8⁺ (2H7 and 2F11) and CD4⁺ (2E1 and 2E6) T-cell clones.