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. 2022 Nov 4;15:3493–3507. doi: 10.2147/JPR.S378876

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© 2022 Anand et al.

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Effect of 2-AG on capsaicin responses. Sample traces from individual neurons showing stable baseline in the presence of vehicle, and rapid increase in intracellular calcium, sustained over several minutes, in response to application of 1 µMol capsaicin (A). Similar traces showing calcium influx in response to added 100 µMol 2-AG, followed by delayed response to capsaicin (B). Delayed capsaicin responses in the presence of 100 µMol 2-AG (C), and 0.1 µMol 2-AG (D). Black arrows indicate application of vehicle (A), or 2-AG (B–D), and red arrow indicates capsaicin application. Scale bars indicate time in seconds (X axis), and 340/380 calcium ratio for response amplitude (Y axis). Graph showing capsaicin response amplitude in the presence of 2-AG at a range of concentrations (n.s. compared with control, but significantly different at 0.01, 0.1 and 1 µMol 2-AG compared with the response amplitude at 10 µMol 2-AG (**P<0.01)) (E). Graph showing highly significant delay in capsaicin responses by several minutes in the presence of 2-AG at all the concentrations tested (***P<0.001) (F).