Figure 4.

PRC2 loss of function elicits the reactivation of hematopoietic transcription programs. (A) Volcano plot analysis of differentially expressed genes in PRC2-altered (n = 14) vs PRC2 WT T-ALL (n = 33). (B) Heatmap representing the unsupervised hierarchical clustering of 47 T-ALL primary samples based on the expression of 1252 differentially expressed genes in PRC2-altered T-ALL (n = 14) vs WT samples (n = 33). The PRC2 status (P), the immunophenotype (I), and the molecular subgroup (M) of each primary sample is indicated. Dendrograms indicate clusters of patients. (C) Bar graphs of GSEA results representing gene sets enrichments in PRC2 WT or altered T-ALL. (D) Bubble plots depicting enrichment in genes associated with epigenetic marks (top) and transcription regulators (bottom). (E) Heatmap indicative of PROGENy pathway perturbation scores for each sample computed on RNA-seq data. The bottom bar plots indicate the mean score for each pathway and their significative enrichment in PRC2 WT or altered T-ALL.