Figure 7. Transcriptomes are altered in the atrium of Dnajb6^+/- mice.

(A) Expression of six calcium homeostasis and ion channel related genes were altered in the Dnajb6^+/- mice right atrium. (B) Expression of four Wnt pathway related genes were altered in the Dnajb6^+/- mice right atrium. (C) Quantitative polymerase chain reaction (qPCR) validation of DE genes listed in A and B, normalized to Gapdh; RNA was extracted from an individual moue right atrium, which was considered a single biological replicate. Samples were collected in triplicate. N=3 mice per group, unpaired student’s t-test.

Figure 7—figure supplement 1. RNA sequencing identifies transcriptome changes in the Dnajb6^+/- mice atrium.

(A) Principal component analysis (PCA) reveals the variance in transcriptome distribution in the Dnajb6^+/- mice and WT controls at 6 months. Each point represents the projections of individual hearts onto principal component (PC). (B) Heatmap of genes differentially expressed in the Dnajb6^+/- mice and WT controls. Each column represents an individual replicate and there are 3 replicates per group. Each row represents an individual gene. The color bar represents relative expression of log-transformed, normalized counts with upregulated genes shown in red and downregulated genes in blue. (C) Volcano plot shows magnitude and significance of genes that altered in distribution in the Dnajb6^+/- mice and WT controls. Genes that significantly downregulated in the Dnajb6^+/- mice are plotted in blue (left) and upregulated are plotted in red (right). (D) Ingenuity Pathway Analysis (IPA) of differentially expressed genes in the Dnajb6^+/- mice and WT controls. Signaling pathways are organized in the order of significance as –log10 of P value.