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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Cell Rep. 2022 Oct 25;41(4):111539. doi: 10.1016/j.celrep.2022.111539

Figure 3. Prolyl-aminoacylation of proS suppressors is less dependent on m1G37.

Figure 3.

(A) Acid-urea gel of cellular prolyl-aminoacylation status of each reconstructed suppressor. An uncharged and un-modified Pro(UGG) is in lane 1, a pair of control tRNA samples of MG1655-trmD-KO grown in m1G37+ and m1G37− conditions in lanes 2 and 3, and the WT MG1655 sample is in lane 4. Data are mean ± SD (n = 3).

(B–D) Kinetics of prolyl-aminoacylation of (B) Pro(UGG), (C) Pro(GGG), and (D) Pro(CGG), showing loss of kcat/Km of the WT enzyme upon removal of m1G37 relative to proS mutants. Data are mean ± SD (n = 3).