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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Cell Rep. 2022 Oct 25;41(4):111539. doi: 10.1016/j.celrep.2022.111539

Figure 4. Dependence on Pro(GGG) for survival of proS suppressors.

Figure 4.

(A) E. coli proK-encoded Pro(CGG) is responsible for reading the CCG codon; the proL-encoded Pro(GGG) is responsible for reading CC[C/U], and the proM-encoded Pro(UGG) is capable of reading CCN due to the cmo5U34 modification.

(B) Construction of a trmD-KO strain with a proS suppressor in MG1655, followed by deletion of proK or proL.

(C) Depletion analysis of Pro(GGG) or Pro(CGG) of the reconstructed trmD-KO strains expressing proS-WT or proS-D98N.

(D) Isoacceptor complementation analysis of reconstructed trmD-KO strains expressing proS-WT or proS-D98N. O/E, over-expression; GGG to UGG, a variant of Pro(GGG) that changed the GGG anticodon to UGG; CAU, tRNAMet(CAU).