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. 2022 Oct 26;16:991403. doi: 10.3389/fnana.2022.991403

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Copyright © 2022 Lund and Hannibal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunostaining showing neuronal somas with intracellular components in the trigeminal ganglion (A–C) with PACAP (green) and CGRP (red). Double immunostaining in panel (A), PACAP (B), and CGRP (C). The left dashed box in panel (A) is magnified in panels (D–F) and shows a cell with colocalized neuropeptides and a CGRP-positive cell. Double immunostaining is seen in panel (D), PACAP (E), and CGRP (F). The right dashed box in panel (A) is magnified in panels (G–I) and illustrates another colocalized cell and a CGRP-positive cell with double immunostaining (G), PACAP (H), and CGRP (I). The dashed box in panel (D) is magnified in panels (J–L) illustrating distinct secretory granules containing either PACAP or CGRP in a cell with colocalization. Double immunostaining in panel (J), PACAP (K), and CGRP (L). Scale bars (A–C) = 30 μm, (D–I) = 5 μm, (J–L) = 2 μm.