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. 2022 Aug 8;12(11):4204–4223. doi: 10.1016/j.apsb.2022.07.023

Figure 6.

Figure 6

PD-L1 regulation capacity of BSA-MHI148@SRF nanoparticles in vitro. (A–C) Expression of the PD-L1 and the p-AMPK protein in MB49 tumor cells revealed by Western blot assay and the corresponding protein quantification by ImageJ after the different treatments for 24 h (n = 3). (D) Representative fluorescence images of PD-L1 expression on the MB49 tumor cell membrane after different treatments, scale bar = 20 μm. (E) Cell surface expression of PD-L1 on MB49 tumor cells determined by flow cytometry (n = 3). (F) Representative fluorescence images of CRT exposure mediated by various treatments, scale bar = 20 μm. (G) Quantification of various treatments mediated CRT exposure (n = 3). Data are demonstrated as mean ± SD. Statistical analysis was performed via the two-tail Student’s t-test. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. (+ L) represented the tumor cells treated with laser irradiation.