Figure 5.

Rumbrins potentially inhibit the early stage of HIV-1 replication. (A) HIV-1 NL4-3luc.R-E- pseudoviruses were used to infect SupT1 cells in the presence of test compounds. The infected cells were lysed followed by the measurement of luciferase activity. EFV and RAL were used as controls; (B) HEK293T cells were transfected with pNL4-3luc.R-E- in the presence of test compounds or DMSO. The resulting virus particles were collected and infected SupT1 cells, followed by measuring luciferase activity to determine viral infectivity. As controls, protease inhibitors (PIs) darunavir (DRV), as well as inactive control EFV were also tested; Data represent the mean ± SD of three independent experiments.