Figure 3.

Broad-spectrum PFT neutralization capability of RM-PL in vitro. (A) Images of RBC hemolysis after RBCs were incubated with Hlα treated with saline, PL, RMV, or RM-PL and (B) the corresponding relative hemolysis (mean ± SD, n = 3). PBS without Hlα and Triton X-100 without Hlα were used as the negative and positive controls, respectively. ∗∗∗P < 0.0001, compared with Hlα+RM-PL. (C–E) Hemolytic activity of free (C) Hlα, (D) LLO and (E) SLO on 5% mouse RBC in vitro. (F–H) Dose-dependent PFT neutralization of RM-PL against (F) Hlα, (G) LLO and (H) SLO determined through RBC hemolysis.