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. 2022 Nov 8;13:6755. doi: 10.1038/s41467-022-34294-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Top panel: Time lapse imaging of a deselected human embryo progressing through the first embryonic mitosis with a lagging chromosome (Embryo 3004iii). Bottom panel: Time lapse imaging of a deselected human embryo of unknown pronuclei status progressing through the first embryonic mitosis with multipolar chromosome segregation (Embryo 3034vii). Chromosomes are visualised using SiR-DNA dye. Z indicates slices shown as a maximum intensity projection. Time in hours:mins, scale bar 20 µm. Blue arrows indicate onset of cleavage furrow ingression. Black arrows indicate polar bodies. All deselected embryos were imaged using a widefield microscope. b Quantification of embryos undergoing the first embryonic mitosis with bipolar chromosome segregation. N numbers are shown within bars. The number of embryos in which micronuclei clearly formed around lagging chromosomes are shown in the fourth bar. P value from a two-sided Fisher’s exact test. c Quantification of embryos undergoing bipolar or multipolar divisions in the first embryonic mitosis. N numbers are shown within bars. As deselected embryos can have varying numbers of pronuclei, this was detailed for embryos dividing with multipolar chromosome segregation (third bar). All egg-share-to-research embryos contain 2 pronuclei. P value from a two-sided Fishers exact test. d Time lapse imaging of a deselected human embryo progressing through the first embryonic mitosis in the presence of lagging chromosomes (white arrows), around which micronuclei form (green arrows). Chromosomes are visualised using SiR-DNA dye. Z indicates slices shown as a maximum intensity projection, time in hours:mins, scale bar 20 µm. (Embryo 3215 v). e Airyscan super-resolution confocal microscopy images of 1PN and 3PN human embryos fixed during the first mitotic division and stained with DAPI, CenpC and tubulin antibodies. White arrows indicate perceived MTOCS/spindle poles. Scale bar 5 µm. Source data are provided as a Source data file.