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. 2022 Nov 9;130:106264. doi: 10.1016/j.bioorg.2022.106264

Fig. 4.

Fig. 4

(A) Fluorescence quenching spectrum SARS-CoV-2 3CLpro (3 μM) in the presence of different concentrations of Thonzonium bromide (0, 6.25, 12.5, 25, 37.5, 50 μM). (B) The Stern-Volmer plots for the fluorescence quenching of 3CLpro by Thonzonium bromide. (C) The static quenching plots for the fluorescence quenching of 3CLpro by Thonzonium bromide. (D) Lineweaver–Burk plots of kinetic data were fitted to obtain the Ki value of 2.62 μM. (E) The inhibition of SARS-CoV-2 3CLpro by Thonzonium bromide in the presence or absence of DTT and GSH. (F) CD spectra of SARS-CoV-2 3CLpro (20 μM) in the presence of different concentrations of Thonzonium bromide (0, 50, 100, 200 μM).