Skip to main content
. 2022 Nov 9;130:106264. doi: 10.1016/j.bioorg.2022.106264

Fig. 6.

Fig. 6

Inhibition of SARS-CoV 3CLpro, MERS-CoV 3CLpro and HCoV-229E 3CLpro by Thonzonium bromide. (A) Multiple-sequence alignment results of 3CLpro sequences of SARS-CoV-2, SARS-CoV, MERS-CoV and HCoV-229E. (B) Purification of four proteases analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Lane M: protein ladder; lane 1: SARS-CoV-2 3CLpro; lane 2: SARS-CoV 3CLpro; lane 3: MERS-CoV 3CLpro; lane 4: HCoV-229E 3CLpro. Representative inhibition curves for Thonzonium bromide against (C) SARS-CoV-2 3CLpro, (D) SARS-CoV 3CLpro, (E) MERS-CoV 3CLpro, and (F) HCoV-229E 3CLpro. Results are expressed as mean ± SD (n = 3).