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. 2022 Nov 8;12:18508. doi: 10.1038/s41598-022-22203-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Synchrotron phase contrast imaging (SR-PCI) with 3D orthographic rendering of an intact left human inner ear. The bony wall of the cochlea was made semi-transparent to permit visualization of the basilar membrane (BM), Rosenthal’s canal (RC) and auditory nerve (AN). The auditory nerve contains approx. 30,000 fibres and their cell bodies are located in a 14.5 mm long spiral bony canal called RC. From there, peripheral neurites spread out to innervate approx. 15,000 hair cells placed on the BM. A probe is shown penetrating the round window (RW) membrane to access the underlying RC. (b) Microradiograph taken following placement of a radio-opaque marker at the presumed site of RC on anatomical dissection; the image confirms precise targeting of RC during dissection.