Skip to main content
. 2022 Sep 1;127(10):1760–1772. doi: 10.1038/s41416-022-01956-7

Fig. 5. ANGPTL4 is essential for hypoxic exosome-induced radioresistance of bystander cells.

Fig. 5

a Representative fluorescence images of exosomes uptake into H1299 cells in a time-dependent manner. Scale bar, 10 μm. b Clonogenic survivals of A549 and H1299 cells that were co-cultured with homologous N2-EXO or O2-EXO for 24 h followed by 6 Gy γ-ray irradiation. c Western blot assay of GPX4, SLC7A11, FTH1, FTL, ANGPTL4 proteins and their relative expression levels in A549 and H1299 cells that were co-cultured with its homologous O2-EXO and N2-EXO with or without γ-ray irradiation. d The transcription and protein levels of ANGPTL4 in bystander A549 cells after co-culturing with its exogenous N2-EXO for indicated times. e Relative level of ANGPTL4 protein in exosomes derived from normoxic A549 and H1299 cells transfected with ANGPTL4-OE or from hypoxic A549 and H1299 cells transfected with sh-ANGPTL4. f Clonogenic survivals of A549 cells that were co-cultured with homologous N2-EXO, N2-sh-A4-EXO, O2-EXO, O2-A4-OE-EXO for 24 h prior to 6 Gy γ-ray irradiation. g Western blot assay of GPX4, FTH1, ANGPTL4 proteins and their relative expression levels in A549 cells that were co-cultured with it homologous N2-EXO, N2-sh-A4-EXO, O2-EXO, O2-A4-OE-EXO or treated with human recombinant ANGPTL4 protein (rANGPTL4) followed by 6 Gy γ-ray irradiation. h Clonogenic survivals of sh-GPX4 transfected A549 cells that were co-cultured with homologous N2-EXO followed by 6 Gy γ-ray irradiation. *P < 0.05; **P < 0.01; ***P < 0.001.