FIG. 7.

Recognition of the MHC class I-restricted βGP1 epitope by lymphocytes primed in vivo in mice by oral vaccination with either MvP101(pAH97), HH104(pAH97), SL7207(pAH97), or carrier alone. Spleen cells from immunized mice were restimulated in vitro for 5 days in the presence of 20 μM βGP1. At the end of the culture period, lymphocytes were tested in a [³H]thymidine retention assay with P815 (open symbols) and βGP1-loaded P815 (solid symbols) as targets (T). Results are means of triplicate wells (one of three independent experiments is shown) and are expressed as [(retained cpm in the absence of effectors {E}) − (experimentally retained cpm in the presence of effectors)/retained cpm in the absence of effectors] × 100. Standard errors of the means were lower than 5%.