Fig. 3.

Drug distribution in rectal compartment 4 h after TAF/EVG administration and rectal cleansing impact. Rectal biopsies were collected at 4 cm (R4), 8 cm (R8), 12 cm (R12), and 25 cm (c) from the anal sphincter 4 h after dosing. Drug concentrations (n = 8/animal) normalised to biopsy weight are plotted as scattered individual values. Rectal inserts were administered 4 cm deep from the anal sphincter either without prior rectal cleansing (1 insert: n = 2, blue or 2 inserts: n = 2, red) and after rectal cleansing (1 insert: n = 3, green or 2 inserts: purple). The second insert was placed at 8 cm from the anal opening. The horizontal line denotes the median. Zero values were given a value of half of the LOQ. Horizontal lines represent statistically significant differences of p < 0.05 (Wilcoxon rank-sum test).